Isolation of equine rotavirus in cell cultures from foals with diarrhea.

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Abstract
A survey of rotaviruses was conducted in 40 fecal samples which were divided into 4 groups according to diarrheal conditions to clarify the relationship between rotavirus and foals with diarrhea. Particles characteristic of rotavirus were directly detected by EM from 5 of 14 fecal samples collected from foals with acute diarrhea. Rotaviruses were isolated from 4 of 5 fecal samples with MA-104 [rhesus monkey embryonic kidney cells] cells and with trypsin treatment and were capable of being successively passaged in these cells. No rotavirus was detected in 26 fecal samples collected from foals with various non-acute diarrheas. In the cross serum neutralization test, 4 isolated strains were antigenically recognized as the same serotype, which differed slightly from BI strain of equine rotavirus and was clearly from Lincoln strain of bovine rotavirus. Thus, rotavirus was closely related only to acute diarrhea in foals. It was confirmed that equine rotavirus in field cases can be isolated in MA-104 cells with trypsin treatment.

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