Helical Sense of Ribbon Assemblies and Splayed Microfibrils of Bacterial Cellulose.

Open Access

1 January 1998

journal article
Published by Society of Fiber Science and Technology Japan in Sen'i Gakkaishi

Vol. 54 (10) , 506-510
https://doi.org/10.2115/fiber.54.10_506

Abstract

Acetobacter xylinum produces cellulose in the form of a twisted thin ribbon assembly. Handedness of the twisted ribbon assembly was examined by transmission electron microscopy (TEM). Special care for correct up-and-down setting of specimens and photographic films was taken to determine the handedness of the twisted assembly by TEM. The ribbon cellulose assemblies were prepared on TEM grids using ATCC 23769 and ATCC 10245 and then shadowed with Pt-Pd. They all showed right-handedness. Splayed microfibrils were prepared from smooth colony of ATCC 23769 by the addition of carboxymethyl cellulose sodium salt (CMC) (DP=80 and DS=0.57) into the culture medium. A bundle of them was twisting together in one body in the right-handed manner.

Keywords

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