Evidence for a tetraglucoside substituent on the lipoteichoic acid of Streptococcus faecium ATCC9790
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 26 (1) , 49-52
- https://doi.org/10.1016/0378-1097(85)90123-5
Abstract
Hydrogen fluoride treatment of [14C-glycerol]lipoteichoic acid synthesized by growing Streptococcus faecium ATCC9790 in the presence of 1,3[14C]glycerol produced five radioactive, water-soluble products which were identified by chromatographic and analytical techniques to be tetraglucosyl glycerol, triglucosyl glycerol, diglucosyl glycerol, monoglucosyl glycerol and unsubstituted glycerol. The percent composition of each varied modestly from culture to culture and ranged between 7 and 8% for the tetra-, 20.5 and 31.2% for the tri-, 11.3 to 23.5% for the di-, 20.9 to 26.8% for the mono-, and 23.1 to 34.8% for the unsubstituted glycerol. The same glucosylated glycerol compounds could be obtained in an in vitro reaction in which a 30 000 ×g particulate enzyme catalyzed the incorporation of [3H]glucose from UDP [3H]glucose into lipoteichoic acid.Keywords
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