Assignment of the 13C N.M.R. spectrum of the Klebsiella K3 serotype polysaccharide by COSY spectroscopy
- 1 January 1989
- journal article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Chemical Communications
- No. 3,p. 167-169
- https://doi.org/10.1039/c39890000167
Abstract
Klebsiella K3 serotype polysaccharide has been biosynthetically enriched by growth on [13C6]glucose and its 13C n. m. r. spectrum has been fully assigned using a phase-sensitive double-quantum-filtered COSY spectrum; analysis of the isotopomer distribution in the COSY cross-peaks shows that some of the glucose is established through the phosphogluconate pathway before incorporation into the polymer.Keywords
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