Molecular characterization of a new human minisatellite that is able to form single‐stranded loops in vitro and is recognized by nuclear proteins

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Abstract
We report the isolation of a new low polymorphic GC‐rich human minisatellite locus (MsH42) that contains different recombination motifs and is homologous to sequences involved in immunoglobulin class‐switching. Furthermore, we show that MsH42 undergoes slipped‐strand mispairing during PCR indicating its ability to generate single‐stranded loops. Specific DNA‐protein complexes were detected in band‐shifting experiments using nuclear extracts from mouse testes and human NC‐37 cells. The possible implications of this minisatellite in recombination events is discussed.