Production and preliminary characterization of RNA-depolymerase from Trichoderma harzianum
- 1 May 1993
- journal article
- Published by Springer Nature in World Journal of Microbiology and Biotechnology
- Vol. 9 (3) , 300-302
- https://doi.org/10.1007/bf00383067
Abstract
Trichoderma harzianum produced RNA-depolymerase with maximum activity after 72 to 120 h of growth. Addition of K2HPO4 repressed enzyme production by the fungus. The optimal activity was at pH 7.8 and 40 to 50°C. The enzyme was stable at pH 3.2 to 9.0 and 80% of activity remained after 60 min at 40°C. EDTA and p-chloromercuribenzoate had no effect on the enzyme activity.Keywords
This publication has 12 references indexed in Scilit:
- Purification and some properties of the extracellular chitinase produced by Trichoderma harzianumEnzyme and Microbial Technology, 1992
- The structural and sequence homology of a family of microbial ribonucleasesTrends in Biochemical Sciences, 1983
- Standardization of assay procedure and some properties of ribonuclease fromaspergillus candidusFolia Microbiologica, 1981
- An absolute method for protein determination based on difference in absorbance at 235 and 280 nmAnalytical Biochemistry, 1980
- The molecular evolution of pancreatic ribonucleaseJournal of Molecular Evolution, 1977
- Partial purification of ribonuclease from Trichoderma koningii.CHEMICAL & PHARMACEUTICAL BULLETIN, 1974
- [Purification of extracellular RNAase of Fusarium semitectum].1972
- Studies on the Characterization of RibonucleasesJournal of Biological Chemistry, 1964
- STUDIES ON THE GROSS STRUCTURE, CROSS-LINKAGES, AND TERMINAL SEQUENCES IN RIBONUCLEASEJournal of Biological Chemistry, 1954
- Notes on sugar determination.1952