Effect of glutathione deficiency on the pool of CoA–glutathione mixed disulfide in Escherichia coli
- 1 May 1981
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 59 (5) , 379-382
- https://doi.org/10.1139/o81-052
Abstract
The formic acid extracts of several glutathione-deficient strains of E. coli were assayed for the presence of the mixed disulfide of CoA and glutathione, CoASSG. Strains deficient in .gamma.-glutamyl-cysteine synthase (EC 6.3.2.2) produced only CoA dimer.Strains deficient in glutathione synthase (EC 6.3.2.3) produced the mixed disulfide of CoA and the .gamma.-glutamyl-cysteine dipeptide. The pool size of total CoA in the cell did not change significantly even in the absence of glutathione.This publication has 12 references indexed in Scilit:
- Levels of coenzyme A – glutathione mixed disulfide in Escherichia coliCanadian Journal of Biochemistry, 1978
- Occurrence of glutathione in bacteriaJournal of Bacteriology, 1978
- Levels of acetyl coenzyme A, reduced and oxidized coenzyme A, and coenzyme A in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of Bacillus megateriumJournal of Bacteriology, 1977
- Identification of a coenzyme A – glutathione disulfide (DSI), a modified coenzyme A disulfide (DSII), and a NADPH-dependent coenzyme A – glutathione disulfide reductase in E. coliCanadian Journal of Biochemistry, 1977
- Most of the Coenzyme A in dormant spores of Bacillus megaterium is in disulfide linkage to proteinBiochemical and Biophysical Research Communications, 1977
- Inhibition of E. coli DNA dependent RNA polymerase by novel nucleotides, DSI and DSIIBiochemical and Biophysical Research Communications, 1976
- Novel nucleotides from E.coli isolated and partially characterizedBiochemical and Biophysical Research Communications, 1976
- Characterization of a nucleotide-peptide from rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- Identity of a Bovine Liver Nucleotide-Peptide with the Unsymmetrical Disulfide of Coenzyme A and GlutathioneJournal of Biological Chemistry, 1965
- THE PURIFICATION OF COENZYME A BY ION EXCHANGE CHROMATOGRAPHYJournal of Biological Chemistry, 1953