A Colorimetric Method for Assay of Erythrocytic Glucose-6-phosphate Dehydrogenase.

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Abstract
The method described, which is independent of a diaphorase, employs phenazine methosulfate as an electron carrier between reduced triphosphopyridine nucleotide formed in the reaction and dichloroindophenol (DCIP). Rate of reduction of DCIP is followed spectrophotometrically at 620 m[mu]. The assay, which gives turnover rates comparable to those obtained with the ultraviolet spectrophoto-metric method, is more sensitive than the latter and any spectrophotom-eter or photoelectric colorimeter may be used.

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