Structure of Milk Fat Triglycerides

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Abstract
The distribution of fatty-acids among the triglycerides of milk fat was studied by thin-layer chromatographic (TLC) technics and lipase hydrolysis. The triglycerides were separated into 2 fractions by thin-layer chromatography on silicic-acid. The fraction with the higher Rf value consisted almost entirely of long-chain triglycerides; the other fraction contained essentially all of the short-chain fatty acids. Treatment of the short-chain triglyceride fraction with sodium-methoxide gave 3 fractions. The triglycerides of these fractions which contained 0, 1 and 2 short-chain fatty-acids per molecule, respectively, demonstrated the nonrandom distribution of the short-chain fatty-acids. A nonrandom distribution of the fatty-acids in the long-chain triglyceride fraction was demonstrated by fractionation and analysis of this fraction by silver nitrate-silicic acid-thin-layer chromatography and lipase hydrolysis. Some 35 triglyceride types were distinguished in milk fat.