Simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci
- 1 May 1980
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 39 (5) , 1070-3
- https://doi.org/10.1128/aem.39.5.1070-1073.1980
Abstract
A simple procedure for rapidly demonstrating small plasmids (less than 10 megadaltons) in oral streptococci is described. Logarithmic-phase, glycine-treated cells from 1.5-ml broth cultures were converted to osmotically fragile forms and lysed with sodium dodecyl sulfate. After the hydrodynamic shearing of host chromosomal deoxyribonucleic acid, such lysates were analyzed by low-voltage agarose gel electrophoresis. Small plasmids, migrating significantly faster than chromosomal deoxyribonucleic acid under such conditions, were readily visualized by ethidium bromide staining.Keywords
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