Effect of Estradiol Benzoate on the Degradation of Insoluble Collagen of Rat Skin1

Abstract
Young female rats were injected with 14C-proline which was incorporated into collagen and converted, in part, to 14C-hydroxyproline (hypro).4 After 2—5 weeks (to allow the bulk of the radioactivity in collagen to be converted to insoluble collagen), we divided the animals into 2 groups. One group was given 50 μg of estradiol benzoate 3 times weekly, while the other served as control. Skin samples obtained by serial biopsy were analyzed for collagen content and 14C-hypro. Estrogen treatment resulted in prolongation of the half-life of collagen 14C-hypro to values averaging greater than twice control values. Collagen concentration was unaffected by estrogen treatment. These results indicate that estrogen treatment retards the degradation of skin collagen. (Endocrinology93: 311, 1973)

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