PURIFIED COLONY-STIMULATING FACTORS ENHANCE THE SURVIVAL OF HUMAN-NEUTROPHILS AND EOSINOPHILS INVITRO - A RAPID AND SENSITIVE MICROASSAY FOR COLONY-STIMULATING FACTORS
- 1 July 1986
- journal article
- research article
- Vol. 68 (1) , 162-166
Abstract
The survival of purified human blood neutrophils and eosinophils was monitored using microwell cultures. Survival was enhanced in cultures containing human or murine colony-stimulating factors (CSFs). The survival of both cell types of enhanced by purified recombinant human granulocyte-macrophage CSF and partially purified preparations of the native molecule, CSF.alpha.. Neutrophil but not eosinophil survival was enhanced by murine granulocyte-CSF and its human analogue CSF.beta.. Eosinophil but not neutrophil survival was enhanced by murine eosinophil differentiation factor (eosinophil CSF). The mature cell survival provided an assay system for CSF that was 102 to 103 more sensitive than the standard assay of stimulation of colony formation in agar cultures and could be completed within 48 hours. These results demonstrated that CSF induced and enhanced survival of mature human neutrophils and eosinophils in vitro. Furthermore, the lineage specificity of purified murine CSFs was retained in their action on human cells.This publication has 27 references indexed in Scilit:
- Effects of GM‐CSF deprivation on precursors of granulocytes and macrophagesJournal of Cellular Physiology, 1982
- Survival of mononuclear phagocytes depends on a lineage-specific growth factor that the differentiated cells selectively destroyCell, 1982
- Separate actions of different colony stimulating factors from human placental conditioned medium on human hemopoietic progenitor cell survival and proliferationJournal of Cellular Physiology, 1981
- HYDROPHOBIC ADSORPTION CHROMATOGRAPHY OF COLONY-STIMULATING ACTIVITIES AND ERYTHROID-ENHANCING ACTIVITY FROM THE HUMAN MONOCYTE-LIKE CELL-LINE, GCT1981
- The persisting (P) cell: histamine content, regulation by a T cell-derived factor, origin from a bone marrow precursor, and relationship to mast cells.Proceedings of the National Academy of Sciences, 1981
- Proliferative capacity, sensitivity to colony stimulating activity and buoyant density: Linked properties of granulocyte-macrophage progenitors from normal human bone marrowLeukemia Research, 1981
- Clonal analysis of proliferation and differentiation of paired daughter cells: action of granulocyte-macrophage colony-stimulating factor on granulocyte-macrophage precursors.Proceedings of the National Academy of Sciences, 1980
- Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell lineJournal of Cellular Physiology, 1980
- A New Method for the Purification of Human Eosinophils and Neutrophils, and a Comparison of the Ability of These Cells to Damage Schistosomula of Schistosoma MansoniThe Journal of Immunology, 1979
- SEPARATION OF FUNCTIONALLY DISTINCT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTORS1979