Colonization of components of a model hot water system by Legionella pneumophila

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Abstract
A model hot water distribution network was seeded with a virulent strain of L. pneumophila serotype 1. Ten weeks after inoculation, components of the system, which include Al discs, Cu, stainless steel, silicone tubing, rubber and glass beads, were examined for colonization by L. pneumophila. The samples were stained with fluorescein-labeled antibodies to the strain and were examined with scanning EM. Colonization which was accompanied by copious quantities of a slime-like debris, was heaviest on the rubber and least on the Cu. Adherence to silicone tubing and stainless steel was observed.