Solid State NMR for Determination of Degree of Acetylation of Chitin and Chitosan

Publisher Website
Google Scholar
Abstract
The degree of acetylation (DA) of various samples has been evaluated by 1H liquid-state NMR and 13C and 15N CP−MAS solid-state NMR over the whole range of DA. A good agreement has been found for all the experiments. The 13C and 15N CP−MAS experiments have permitted the evaluation of the chitin DA and content in the structural polysaccharides in a fungus named Aspergillus niger. The fungus structural carbohydrates mainly consist in pure chitin associated with glucans. Comparison of the 13C CP−MAS spectra with standard (1→3)-β-d-glucans strongly suggests that chitin and glucans are linked via covalent bonds.