Localization of the binding site for the human high-affinity Fc receptor on IgG
- 1 April 1988
- journal article
- Published by Springer Nature in Nature
- Vol. 332 (6164) , 563-564
- https://doi.org/10.1038/332563a0
Abstract
A major pathway in the clearance of pathogens involves the coating of the pathogen with specific antibodies, and the binding of the antibody Fc region to cell receptors. This can trigger engulfment of the pathogen by phagocytes or lysis by killer cells. By oligonucleotide site-directed mutagenesis we have engineered a single amino acid change in a mouse IgG2b antibody (Glu 235----Leu) which now enables the antibody to bind to the FcRI (high affinity) receptor on human monocytes with a 100-fold improvement in affinity. This indicates that Leu 235 is a major determinant in the binding of antibody to FcRI and that the receptor may interact directly with the region linking the CH2 domain to the hinge. Tailoring the affinity of antibodies for cell receptors could help dissect their role in clearing pathogen.Keywords
This publication has 19 references indexed in Scilit:
- The use of anti-IgG monoclonal antibodies in mapping the monocyte receptor site on IgGMolecular Immunology, 1986
- Human leukocyte IgG Fc receptorsImmunology Today, 1986
- Is IgM-like dislocation a common feature of antibody function?Immunology Today, 1986
- Construction of novel antibodies by use of DNA transfection: design of plasmid vectorsPhilosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences, 1986
- Human IgG1 and its Fc fragment bind with different affinities to the Fc receptors on the human U937, HL-60 and ML-1 cell linesMolecular Immunology, 1985
- The Binding of Human Immunoglobulin G1 Monomer and Small, Covalently Cross-Linked Polymers of Immunoglobulin G1 to Human Peripheral Blood Monocytes and Polymorphonuclear LeukocytesJournal of Clinical Investigation, 1982
- An IgG2a-producing variant of an IgG2b-producing mouse myeloma cell line. Structural studies on the Fc region of parent and variant heavy chainsBiochemistry, 1978
- Variability of Interchain Binding of Immunoglobulins: Interchain Bridges of Mouse IgG1Nature, 1970
- Variations in the S—S bridges of immunoglobins G: Interchain disulphide bridges of γG3 myeloma proteinsJournal of Molecular Biology, 1968
- Disulphide Bridges of Immunoglobin G1 Heavy ChainsNature, 1967