DIFFERENCES IN LIPID-COMPOSITION OF DOXORUBICIN-SENSITIVE AND DOXORUBICIN-RESISTANT P388 CELLS

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 68  (4) , 637-641
Abstract
An anthracycline-resistant subline of P388 murine leukemia cells, P388/ADR, has an altered total lipid content and composition when compared to the parent line. When specific lipids of the cell lines are compared, P388/ADR cells contain .apprx. 3.6 times the amount of triglycerides as P388 cells. Although no differences are noted in the amounts of unesterified cholesterol or total phospholipids in the 2 cell lines, they differ in specific phospholipid patterns. P388/ADR cells contain relatively less phosphatidylcholine and more sphingomyelin than drug-sensitive cells. No differences are observed in the content of the phosphatidylethanolamine and cardiolipin. The difference in phosphatidylcholine/sphingomyelin ratio (5.57 for ADR-sensitive cells and 3.28 for ADR-resistant cells) may account for the previously observed difference in plasma membrane lipid structural order between these cell lines. Measurements of the relative activity of phosphocholine transferase in the 2 lines, using the rate of incorporation of 3H-choline into phosphatidylcholine, indicate that lower phosphocholine transferase activity in P388/ADR cells may account for the observed changes in cellular lipid and phospholipid composition.