Hydrolysis of olive oil by pancreatic lipase in biphasic organic-aqueous system
- 1 January 1989
- journal article
- Published by Institute of Organic Chemistry & Biochemistry in Collection of Czechoslovak Chemical Communications
- Vol. 54 (10) , 2809-2813
- https://doi.org/10.1135/cccc19892809
Abstract
Six ethylene oxide-based surfactants were tested for their suitability to mediate lipase action on olive oil in a water-organic biphasic system. In Slovasol EL only it was possible by lipase to catalyze hydrolysis of the substrate over a wide range of isooctane and water content in the system. The Michaelis-Menten parameters of lipase measured for this system are comparable with those measured in a reversed emulsion of water and oil-isooctane solution. The hydrolysis rate was not increased, however, in the presence of isooctane.Keywords
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