Determination of Hydrogen Peroxide by Flow Injection Analysis with Aryl Oxalate-Sulforhodamine 101 Chemiluminescence

Abstract
A flow injection analysis (FIA) method for the determination of hydrogen peroxide by aryl oxalate chemiluminescence detection was studied. The analyte was detected by using sulforhodamine 101 as a new fluorophore and bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl]oxalate (TDPO) in imidazole buffer (pH 7.0)-acetonitrile. The detection limit was 3.0 × 10−9 M. The relative standard deviation (n=6) for 1.0 × 10−6 M hydrogen peroxide was 2.8%. This FIA method was shown to be 20 to 2500 times more sensitive than the previous FIA method with aryl oxalate chemiluminescence.