Homology Between the MPB70 and MPB83 Proteins of Mycobacterium bovis BCG
- 1 July 1995
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 42 (1) , 46-51
- https://doi.org/10.1111/j.1365-3083.1995.tb03624.x
Abstract
Isolation of MPB83 from Mycobacterium bovis BCG Tokyo culture fluid is described. MPB70 and MPB83 have similar molecular mass as judged by SDS-PAGE but differ in isoelectric points. Peptides isolated after CNBr cleavage of MPB83 revealed extensive homology as well as distinct differences from corresponding parts of the amino acid sequence deduced from the mpb70 gene cloned by Terasaka et al. Antibodies produced by immunization with MPB70 and MPB83 had distinctly different fine specificity revealing cross-reactivity between the proteins. These findings indicate that two distinct, homologous genes code for these proteins. Sensitization with live BCG Tokyo also induced T cell responses to MPB83 with development of delayed type hypersensitivity in guinea pigs.Keywords
This publication has 40 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Characterization of the Gene Encoding the MPB51, One of the Major Secreted Protein Antigens of Mycobacterium bovis BCG, and Identification of the Secreted Protein Closely Related to the Fibronectin Binding 85 ComplexScandinavian Journal of Immunology, 1995
- Production and characterization of a monoclonal badger anti-immunoglobulin G and its use in defining the specificity of Mycobacterium bovis infection in badgers by Western blotVeterinary Immunology and Immunopathology, 1994
- Purification of MPB70 and Production of Specific Monoclonal AntibodiesHybridoma, 1992
- A Family of Cross-Reacting Proteins Secreted by Mycobacterium tuberculosisScandinavian Journal of Immunology, 1992
- Tuberculosis in domesticated red deer: Comparison of purified protein derivative and the specific protein MPB70 for in vitro diagnosisResearch in Veterinary Science, 1991
- A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosisJournal of General Microbiology, 1991
- Mapping of the T and B cell epitopes of the Mycobacterium bovis protein, MPB70Immunology & Cell Biology, 1990
- The Complete Amino‐Acid Sequence of Non‐Immunolobulin Amyloid Fibril Protein AS in Rheumatoid ArthritisEuropean Journal of Biochemistry, 1974
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964