Purification of a factor from Ehrlich ascites tumor cells specifically stimulating RNA polymerase II

Publisher Website
Google Scholar
Abstract
A factor stimulating RNA polymerase II from Ehrlich ascites tumor cells [mouse] was purified. The final preparation appeared almost homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and had a MW of 38,000. The endonuclease activity of about 10 .mu.g of purified factor, if any, was well below the 10-5 .mu.g equivalent of pancreatic DNase, indicating that the stimulation of RNA synthesis by this factor was not due to contaminating endonuclease. This factor specifically stimulated RNA polymerase II on native DNA as template and did not affect RNA polymerase I at all. The molecular size of RNA synthesized in the presence of this factor increased markedly compared with that synthesized by RNA polymerase II alone.

This publication has 1 reference indexed in Scilit: