Superantigen-reactive CD4+ T cells are required to stimulate B cells after infection with mouse mammary tumor virus.
Open Access
- 1 February 1993
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 177 (2) , 359-366
- https://doi.org/10.1084/jem.177.2.359
Abstract
Superantigens are defined by their ability to stimulate a large fraction of T cells via interaction with the T cell receptor (TCR) V beta domain. Endogenous superantigens, classically termed minor lymphocyte-stimulating (Mls) antigens, were recently identified as products of open reading frames (ORF) in integrated proviral copies of mouse mammary tumor virus (MMTV). We have described an infectious MMTV homologue of the classical endogenous superantigen Mls-1a (Mtv-7). The ORF molecules of both the endogenous Mtv-7 and the infectious MMTV(SW) interact with T cells expressing the TCR V beta 6, 7, 8.1, and 9 domains. Furthermore, the COOH termini of their ORF molecules, thought to confer TCR specificity, are very similar. Since successful transport of MMTV from the site of infection in the gut to the mammary gland depends on a functional immune system, we were interested in determining the early events after and requirements for MMTV infection. We show that MMTV(SW) infection induces a massive response of V beta 6+ CDC4+ T cells, which interact with the viral ORF. Concomitantly, we observed a B cell response and differentiation that depends on both the presence and stimulation of the superantigen-reactive T cells. Furthermore, we show that B cells are the main target of the initial MMTV infection as judged by the presence of the reverse-transcribed viral genome and ORF transcripts. Thus, we suggest that MMTV infection of B cells leads to ORF-mediated B-T cell interaction, which maintains and possibly amplifies viral infection.Keywords
This publication has 31 references indexed in Scilit:
- Two monoclonal rat antibodies with specificity for the beta-chain variable region V beta 6 of the murine T-cell receptor.Proceedings of the National Academy of Sciences, 1988
- Interferon-γ and B Cell Stimulatory Factor-1 Reciprocally Regulate Ig Isotype ProductionScience, 1987
- Proteins Antigenically Related to Peptides Encoded by the Mouse Mammary Tumour Virus Long Terminal Repeat Sequence Are Associated with Intracytoplasmic A ParticlesJournal of General Virology, 1987
- Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.The Journal of Immunology, 1985
- BALB.D2-Mlsa-A new congenic mouse strainTransplantation, 1984
- Nucleotide sequence of the 5′ noncoding region and part of thegaggene of mouse mammary tumor virus; identification of the 5′ splicing site for subgenomic mRNAsNucleic Acids Research, 1983
- Alloreactive cloned T cell lines. II. Polyclonal stimulation of B cells by a cloned helper T cell line.The Journal of Immunology, 1981
- Specific T helper cells that activate B cells polyclonally. In vitro enrichment and cooperative function.The Journal of Experimental Medicine, 1980
- Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*Immunological Reviews, 1979
- Mouse strain and breeding stimulation as factors influencing the effect of thymectomy on mammary tumorigenesis.1970