The development of fluorescence intensity standards
- 1 March 2001
- journal article
- Published by National Institute of Standards and Technology (NIST) in Journal of Research of the National Institute of Standards and Technology
- Vol. 106 (2) , 381-9
- https://doi.org/10.6028/jres.106.015
Abstract
The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the inability to make comparable fluorescence intensity measurements across laboratories. NIST recognizes the need to develop and provide primary fluorescence intensity standard (FIS) reference materials to the scientific and technical communities involved in these assays. The critical component of the effort will be the cooperation between the Federal laboratories, the manufacturers, and the technical personnel who will use the fluorescence intensity standards. We realize that the development and use of FIS will have to overcome many difficulties. However, as we outline in this article, the development of FIS is feasible.Keywords
This publication has 9 references indexed in Scilit:
- Flow cytometric quantitation of immunofluorescence intensity: Problems and perspectivesCytometry, 1998
- Standardizing flow cytometry: A classification system of fluorescence standards used for flow cytometryCytometry, 1998
- Quantitation of CD38 expression using QuantiBRITE? beadsCytometry, 1998
- Performance of calibration standards for antigen quantitation with flow cytometryCytometry, 1998
- Standardizing flow cytometry: construction of a standardized fluorescence calibration plot using matching spectral calibratorsCytometry, 1996
- [15] Covalent labeling of proteins and nucleic acids with fluorophoresPublished by Elsevier ,1995
- Development of Clinical Standards for Flow CytometryAnnals of the New York Academy of Sciences, 1993
- Controls for Flow Cytometers in Hematology and Cellular ImmunologyAnnals of the New York Academy of Sciences, 1986
- A new standard fluorescence microsphere for quantitative flow cytometryJournal of Immunological Methods, 1985