Measurement of Slowly Exchanging 45Ca in Rat Aorta without Using EGTA or Lanthanum and Its Application to Quantify the Effects of the Calcium Entry Blockers Nifedipine and Verapamil
- 1 January 1988
- journal article
- research article
- Published by S. Karger AG in Pharmacology
- Vol. 36 (2) , 84-90
- https://doi.org/10.1159/000138363
Abstract
In rat aortic strips a method was developed to measure a fraction of slowly exchanging 45Ca, which correlates with contraction and the cytosolic Ca pool that is enhanced by K+-induced depolarization. In this method no EGTA or lanthanum are used, but the strips are washed for 45 min with a Tyrode solution at 4°C. The K+ depolarization induced increase in 45Ca and contraction was concentration-dependently inhibited by verapamil and nifedipine. Since lanthanum and EGTA affect cellular membranes, this method may allow a more physiological approach to the measurements of slowly exchanging 45Ca.Keywords
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