Preparative Purification of Dipeptidyl Peptidase IV
- 1 June 1991
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 21 (2-3) , 141-150
- https://doi.org/10.1080/10826069108018009
Abstract
Dipeptidyl-Peptidase IV was purified from pig kidney by ammonium sulfate fractionation, gel filtration, QAE-cellulose chromatography and affinity columns with Gly-Pro- and Concanavalin A-Sepharose. The specific activity of the purified enzyme is 41.8 units/mg. Polyacrylamide gel electrophoresis and silver staining show a single band. The enzyme preparation is free of aminopeptidase and dipeptidase activity, proved fluorimetrically and by gas chromatography/mass spectrometry. The most important procedure for removal of contaminating enzyme activities is a stepwise NaCl-gradient on a QAE-ZetaPrep ion exchange disk.Keywords
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