Effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis: Theoretical calculation and experimental evaluation
- 1 January 1995
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 16 (1) , 516-524
- https://doi.org/10.1002/elps.1150160185
Abstract
The effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis (CE) is studied. A system for predictions of peptide migration in CE (SPPMCE) developed in our laboratory has been tested in a wide range of pH and buffer concentrations. The SPPMCE consists of a computer program for calculating peptide pKa values, an equation which relates peptide structures to their electrophoretic mobilities and a coupled computer program for the prediction of electropherograms. More than 25 different buffers have been employed, covering a pH range of 2–11 and a concentration range of 5–100 mM. Results from experiments are compared with the theoretical predictions. Good agreement is observed, which confirms the utility of the SPPMCE and allows fast and easy optimization of peptide separations in CE, with nothing more than the amino acid sequence of the linear peptide as the input.Keywords
This publication has 39 references indexed in Scilit:
- Optimization of capillary electrophoresis of mixtures of basic peptides and comparison with HPLCAnalytical Chemistry, 1993
- Capillary Electrophoresis Separations of Peptides: Practical Aspects and ApplicationsPublished by Elsevier ,1992
- Correlation of electrophoretic mobilities from capillary electrophoresis with physicochemical properties of proteins and peptidesAnalytical Biochemistry, 1991
- Capillary electrophoresis of peptides : Analysis of adrenocorticotropic hormone-related fragmentsJournal of Chromatography A, 1991
- Method optimization in capillary zone electrophoretic analysis of hGH tryptic digest fragmentsJournal of Chromatography A, 1990
- Recent advances in capillary electrophoresis of proteins, peptides and amino acidsElectrophoresis, 1990
- Capillary zone electrophoresis of peptide fragments from trypsin digestion of biosynthetic human growth hormoneJournal of Chromatography A, 1989
- High-sensitivity peptide mapping by capillary zone electrophoresis and microcolumn liquid chromatography using immobilized trypsin for protein digestionAnalytical Chemistry, 1989
- Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptidesAnalytical Chemistry, 1989
- Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresisAnalytical Biochemistry, 1988