Distal regulatory regions restrict the expression of cis ‐linked genes to the tapetal cells
- 17 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 517 (1-3) , 13-18
- https://doi.org/10.1016/s0014-5793(02)02557-7
Abstract
The oleosin glycine‐rich protein genes Atgrp‐6, Atgrp‐7, and Atgrp‐8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis‐regulatory regions involved in the tissue‐specific gene expression were investigated by fusing different segments of the gene cluster to the uidA reporter gene. Common distal regulatory regions were identified that coordinate expression of the sequential genes. At least two of these genes were regulated spatially by proximal and distal sequences. The cis‐acting elements (122 bp upstream of the transcriptional start point) drive the uidA expression to floral tissues, whereas distal 5′ upstream regions restrict the gene activity to tapetal cells.Keywords
This publication has 41 references indexed in Scilit:
- Role of lipid bodies and lipid-body proteins in seeds and other tissuesJournal of Plant Physiology, 2001
- Gene regulation and deregulation: a β globin perspectiveBlood Reviews, 2000
- Stopped at the border: boundaries and insulatorsCurrent Opinion in Genetics & Development, 1999
- Identification of a Nuclear Factor-I Family Protein-binding Site in the Silencer Region of the Cartilage Matrix Protein GenePublished by Elsevier ,1995
- Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for FunctionPlant Cell, 1993
- The TACPyAT repeats in the chalcone synthase promoter of Petunia hybrida act as a dominant negative cis‐acting module in the control of organ‐specific expressionThe Plant Journal, 1992
- In‐situ enzyme histochemistry on plastic‐embedded plant material. The development of an artefact‐free β‐glucuronidase assayThe Plant Journal, 1992
- Sequence Requirements of the 5-Enolpyruvylshikimate-3-Phosphate Synthase 5'-Upstream Region for Tissue-Specific Expression in Flowers and SeedlingsPlant Cell, 1990
- Differential expression of five Arabidopsis genes encoding glycine-rich proteins.Plant Cell, 1990
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962