MutM, a protein that prevents G C→T A transversions, is formamidopyrimidine-DNA glycosylase
Open Access
- 1 January 1991
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (13) , 3629-3632
- https://doi.org/10.1093/nar/19.13.3629
Abstract
We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase). An overproducing clone of Fapy-DNA glycosylase complements the original mutM strain that had been isolated after EMS mutagenesis. Thus, we conclude that MutM is actually Fapy-DNA glycosylase. mutM has previously been characterized as a mutator strain that leads specifically to G C-T A transversions. This in vivo characterization correlates well with the mutagenic potential of one of the lesions Fapy-DNA glycosylase removes, 8-oxo-7, 8-dihydro-2′-deoxyguanine (8-OxodG).Keywords
This publication has 23 references indexed in Scilit:
- NMR structural studies of the ionizing radiation adduct 7-hydro-8-oxodeoxyguanosine (8-oxo-7H-dG) opposite deoxyadenosine in a DNA duplex. 8-Oxo-7H-dG(syn).cntdot.dA(anti) alignment at lesion siteBiochemistry, 1991
- Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodGNature, 1991
- mutA and mutC: two mutator loci in Escherichia coli that stimulate transversions.Proceedings of the National Academy of Sciences, 1990
- Cloning of genes interruptep by Tn10 derivatives using antibiotic-resistance-carrying M13mp bacteriophagesGene, 1990
- Mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genomeBiochemistry, 1990
- Ring-opened 7-methylguanine residues in DNA are a block to in vitro DNA synthesisNucleic Acids Research, 1988
- The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversions.Proceedings of the National Academy of Sciences, 1988
- MUTAGENESIS BY APURINIC/APYRIMIDINIC SITESAnnual Review of Genetics, 1986
- Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from Escherichla coliNucleic Acids Research, 1979
- A Factor (or Mutator Gene) Influencing Mutation Rates in Escherichia ColiProceedings of the National Academy of Sciences, 1954