Activation of formylmethanofuran synthesis in cell extracts of Methanobacterium thermoautotrophicum
Open Access
- 1 March 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (3) , 1423-1427
- https://doi.org/10.1128/jb.171.3.1423-1427.1989
Abstract
In cell extracts of Methanobacterium thermoautotrophicum, formylmethanofuran (formyl-MFR) synthesis (an essential CO2 fixation reaction that is an early step in CO2 reduction to methane) is subject to a complex activation that involves a heterodisulfide of coenzyme M and N-(7-mercaptoheptanoyl)threonine O3-phosphate (CoM-S-S-HTP). In this paper we report that titanium(III) citrate, a low-potential reducing agent, stimulated CO2 reduction to methane and activated formyl-MFR synthesis in cell extracts. Titanium(III) citrate functioned as the sole source of electrons for formyl-MFR synthesis and enabled this reaction to occur independently of CoM-S-S-HTP. In addition, CoM-S-S-HTP was found to activate an unknown electron carrier that reduced metronidazole. The activation of formyl-MFR synthesis by CoM-S-S-HTP may involve the activation of a low-potential electron carrier.This publication has 37 references indexed in Scilit:
- Methanobacterium thermoautotrophicum contains a soluble enzyme system that specifically catalyzes the reduction of the heterodisulfide of coenzyme M and 7‐mercaptoheptanoylthreonine phosphate with H2FEBS Letters, 1988
- The final step in methane formationEuropean Journal of Biochemistry, 1988
- Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate is a product of the methylreductase reaction in MethanobacteriumBiochemical and Biophysical Research Communications, 1987
- The role of 7-mercaptoheptanoylthreonine phosphate in the methylcoenzyme M methylreductase system from Methanobacterium thermoautotrophicumBiochemical and Biophysical Research Communications, 1987
- Coenzyme F420 dependence of the methylenetetrahydromethanopterin dehydrogenase of MethanobacteriumthermoautotrophicumBiochemical and Biophysical Research Communications, 1985
- Unusual coenzymes of methanogenesisTrends in Biochemical Sciences, 1985
- Methanofuran (carbon dioxide reduction factor), a formyl carrier in methane production from carbon dioxide in MethanobacteriumBiochemistry, 1985
- 5,10-Methenyl-5,6,7,8-tetrahydromethanopterin, a one-carbon carrier in the process of methanogenesisBiochemistry, 1984
- Structure of methanofuran, the carbon dioxide reduction factor of Methanobacterium thermoautotrophicumJournal of the American Chemical Society, 1984
- Stimulation of CO2 reduction to methane by methyl-coenzyme M in extracts of MethanobacteriumBiochemical and Biophysical Research Communications, 1977