PHYTOCHROME INTERMEDIATES IN VIVO‐I. EFFECTS OF TEMPERATURE, LIGHT INTENSITY, WAVELENGTH AND OXYGEN ON INTERMEDIATE ACCUMULATION
- 1 August 1973
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 18 (2) , 139-144
- https://doi.org/10.1111/j.1751-1097.1973.tb06404.x
Abstract
Abstract— Accumulation of weakly absorbing phytochrome intermediates has been demonstrated in Pisum epicotyl tissue under conditions of pigment cycling using a quasi‐continuous measuring spectrophotometer. An action spectrum shows 690–700 nm to be the most efficient wavelength range in this process. Difference spectra for the decay of intermediates maintained by 690 nm light show that, if the experiment is done at 0°C, only Pfr is formed. At – 11°C, intermediates decaying to Pr can also be observed. At – 20°C, Pr is produced as well as a pigment with peak absorption at 710nm. Kinetic analysis of intermediate decay at – 11°C reveals that at least two intermediates are maintained by 690 nm light. The level of intermediate maintained by incandescent light at 0°C was 25% higher in air than in nitrogen.This publication has 19 references indexed in Scilit:
- PHYTOCHROME INTERMEDIATES IN VIVO– III KINETIC ANALYSIS OF INTERMEDIATE REACTIONS AT LOW TEMPERATUREPhotochemistry and Photobiology, 1973
- PHYTOCHROME INTERMEDIATES IN VIVO–II. CHARACTERISATION OF INTERMEDIATES BY DIFFERENCE SPECTROPHOTOMETRYPhotochemistry and Photobiology, 1973
- Tension Transients after Quick Release in Rat and Frog Skeletal MusclesNature, 1972
- Chemical evidence for conformational differences between the red- and far-red-absorbing forms of oat phytochromeBiochemistry, 1972
- Catalysis of the phytochrome dark reaction by reducing agentsBiochemistry, 1971
- Some Spectral Properties of Pea Phytochrome in Vivo and in VitroPlant Physiology, 1970
- STABILIZATION OF PHYTOCHROME INTERMEDIATES BY LOW TEMPERATURE*Photochemistry and Photobiology, 1968
- Low-temperature action spectra for transformations of photoperiodic pigmentsBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- Photoreversible pigment transformations in etiolated plantsBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- ACTTON SPECTRA OF PHYTOCHROME IN VITRO*Photochemistry and Photobiology, 1964