Reductive dehydroxylation of 4‐hydroxybenzoyl‐CoA to benzoyl‐CoA in a denitrifying, phenol‐degrading Pseudomonas species
- 17 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2) , 237-240
- https://doi.org/10.1016/0014-5793(89)81461-9
Abstract
The initial reactions in anaerobic degradation of phenol to CO2 have been studied in vitro with a denitrifying Pseudomonas strain grown with phenol and nitrate in the absence of molecular oxygen. Phenol has been proposed to be carboxylated to 4-hydroxybenzoate [(1987) Arch. Microbiol. 148, 213–217]. 4-Hydroxybenzoate was activated to 4-hydroxybenzoyl-CoA by a coenzyme A ligase. Cell extracts also catalyzed the reductive dehydroxylation of 4-hydroxybenzoyl-CoA to benzoyl-CoA with reduced benzyl viologen as electron donor. This enzyme, benzoyl-CoA:(acceptor) 4-oxidoreductase (hydroxylating) (EC 1.3.99.-), has not been reported before. The data suggest that phenol and 4-hydroxybenzoate are anaerobically metabolized by this strain via benzoyl-CoA.Keywords
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