Simplified Method for Determination of Total Adrenal Cholesterol.
- 1 October 1954
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 87 (1) , 48-50
- https://doi.org/10.3181/00379727-87-21281
Abstract
The method of Zlatkis, Zak and Boyle (J. Lab. and Clin. Med., 1953, 41: 486) for the direct determination of serum cholesterol was adapted to determination of total adrenal cholesterol. The values thus obtained do not differ significantly from those obtained by the method of Sperry and Webb in normal, hypophysectomized and cold stressed rats. The time required to analyze 10 samples and 5 standards by the Zlatkis, Zak and Boyle procedure requires less than 1 hour compared to the 2 days usually necessary when methods based on the Liebermann-Burchard reaction are utilized.Keywords
This publication has 1 reference indexed in Scilit:
- A REVISION OF THE SCHOENHEIMER-SPERRY METHOD FOR CHOLESTEROL DETERMINATIONJournal of Biological Chemistry, 1950