The Histochemical Demonstration of Hemoglobin in Blood Cells and Tissue Smears

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Abstract
The presence of Hb in cells may be demonstrated by means of the following specific staining reaction. A dried blood smear is flooded with a 1% soln. of benzidine in absolute methyl ale. and allowed to stand 1 min. The benzidine soln. is replaced with a 25% soln. of Superoxol in 70% ethyl ale. After 1 1/2 min. the slide is washed in distilled water for 15 sec. The slide is then dried and mounted in neutral Canada balsam. Structures containing Hb are colored dark brown. No other substance is stained. Wright''s stain may be used subsequent to the benzidine procedure.

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