Nuclear proteins in Drosophila melanogaster cells after heat shock and their binding to homologous DNA
Open Access
- 11 March 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (5) , 943-955
- https://doi.org/10.1093/nar/8.5.943
Abstract
After 5 minutes heat shock at 37°C Drosophila melanogaster Kc-cell nuclear proteins were extracted with 0.4M NaCl and compared by SDS gel electrophoresis with extracts from cells grown at 25°C. Two proteins (39 000 and 46 000) were only found in heat shock nuclei. Reconstitution with total Drosophila DNA or a DNA fragment from the heat inducible locus 87A/C covalently coupled to sepharose was performed. In the presence of calf thymus competitor DNA these proteins and also others of lower molecular weight showed preferential binding to the homologous DNA.Keywords
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