Extension of DNA triple helix formation to a neighbouring (AT) n site
- 20 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 360 (1) , 21-25
- https://doi.org/10.1016/0014-5793(95)00069-l
Abstract
We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6 · (AT)6T11, using oligonucleotides designed to form parallel T · AT and G · TA triplets. We find that, although (TG)6 does not form a complex with (AT)6 · (AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.Keywords
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