Cryopreservation of echinoderm sperm
- 1 June 1973
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 51 (6) , 666-669
- https://doi.org/10.1139/z73-100
Abstract
A simple method for the cryopreservation of sea urchin, sand dollar, and starfish sperm is described. Using dimethyl sulfoxide (DMSO) as the protective agent, sperm were stored up to 30 days at — 196 °C. These preparations all retained some motility after thawing. Some samples of thawed sea urchin sperm could achieve a level of egg fertilization equivalent to that achieved by fresh, unfrozen sperm, provided that large doses of the thawed sperm were used. Attempts to cryopreserve fucoid sperm by the same method were unsuccessful.Keywords
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