Expression of Recombinant Envelope Protein of Japanese Encephalitis Virus YL Strain in Escherichia coli Possesses Hemagglutination Activity
- 1 March 2004
- journal article
- Published by Springer Nature in Virus Genes
- Vol. 28 (2) , 215-221
- https://doi.org/10.1023/b:viru.0000016861.78378.9b
Abstract
The nucleotide sequence of glycoprotein E of YL vaccine strain was cloned, sequenced and expressed in E. coli. Phylogenetic analysis of envelope (E) amino acid sequences of 18 JEVs in GenBank showed that the vaccine strain YL closer to the virulent strain HVI which is a Taiwanese isolate. We found only two amino acid mutations (K-138 and G-389) of E protein might lead viral attenuation in YL. In this study, we used pRSET vector system to construct three recombinant plasmids (pRSET/F1R1, pRSET/F2R2 and pRSET/F1R2), which encoded and expressed different or overlapping amino acid region of E protein. The antigenicity and hemagglutination activity of these recombinant proteins were examined by western blotting and hemagglutination test, respectively. Our results demonstrated that the recombinant protein of pRSET/F1R2 possesses predominant antigenicity and hemagglutination activity.Keywords
This publication has 23 references indexed in Scilit:
- Cloning and Sequencing of Complete cDNA of Japanese Encephalitis Virus YL Strain in TaiwanVirus Genes, 2003
- A ‘minimal’ approach in design of flavivirus infectious DNAVirus Research, 2001
- Amplification and cloning of the full-length genome of Japanese encephalitis virus by a novel long RT-PCR protocol in a cosmid vectorJournal of Virological Methods, 2001
- Development of a simplified assay for the detection of neutralizing antibodies to Japanese encephalitis virusJournal of Virological Methods, 2001
- Phylogenetic analysis suggests only one serotype of Japanese encephalitis virusVaccine, 2000
- Molecular characterization of the Japanese encephalitis virus representative immunotype strain JaGAr 01Virus Research, 1999
- Japanese encephalitis virus antigenic variants with characteristic differences in neutralization resistance and mouse virulenceVirus Research, 1997
- Generation and Characterization of Organ-Tropism Mutants of Japanese Encephalitis Virusin Vivoandin VitroVirology, 1996
- The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolutionNature, 1995
- Mutations in the envelope protein of japanese encephalitis virus affect entry into cultured cells and virulence in miceVirology, 1992