The influence of the medium on the production of bacterial gelatinase

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Abstract
Gelatinase was demonstrated by following the change in viscosity of a mixture of gelatin and sterile centrifugate from the medium in which the organism had been grown. In a medium containing no added salts, it made little difference whether the source of N was NH4Cl or any of the following compounds: alanine, arginine, asparagine, tyrosine, glutamic acid or glycine, except that better growth was obtained with some than with others. A mixture of Ca and Mg salts stimulated gelatinase production. Mg salts often inhibited gelatinase production, even though they might stimulate growth, while Ca salts had but little growth-stimulating action, but usually stimulated gelatinase production.

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