Determination of 4‐hydroxytamoxifen in mouse plasma in the pg/mL range by gradient capillary liquid chromatography/tandem mass spectrometry
- 30 January 2001
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 15 (4) , 297-303
- https://doi.org/10.1002/rcm.225
Abstract
Capillary high‐performance liquid chromatography (HPLC; 300 µm i.d.) coupled to tandem mass spectrometry has been used to determine the concentration of 4‐hydroxytamoxifen in mouse plasma in the pg/mL range following the administration of Tamoxifen. A limit of quantification (LOQ) of 100 pg/mL was achieved using only 25 µL of plasma. The on‐column sensitivity was determined to be 100 fg. The column performance was determined isocratically before and after the assay and showed only a 15% reduction in performance after 70 injections of plasma extract. No significant peak band broadening was observed due to the mass spectrometer interface using a standard TurboIonspray source. Copyright © 2001 John Wiley & Sons, Ltd.Keywords
This publication has 10 references indexed in Scilit:
- The use of capillary high performance liquid chromatography with electrospray mass spectrometry for the analysis of small volume blood samples from serially bled mice to determine the pharmacokinetics of early discovery compoundsRapid Communications in Mass Spectrometry, 1999
- Ultra-high flow rate capillary liquid chromatography with mass spectrometric detection for the direct analysis of pharmaceuticals in plasma at sub-nanogram per millilitre concentrationsRapid Communications in Mass Spectrometry, 1999
- The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of pharmaceuticals in biofluidsRapid Communications in Mass Spectrometry, 1999
- The rapid identification of drug metabolites using capillary liquid chromatography coupled to an ion trap mass spectrometerRapid Communications in Mass Spectrometry, 1999
- The need for adequate chromatographic separation in the quantitative determination of drugs in biological samples by high performance liquid chromatography with tandem mass spectrometryRapid Communications in Mass Spectrometry, 1999
- Optimisation and routine use of generic ultra-high flow-rate liquid chromatography with mass spectrometric detection for the direct on-line analysis of pharmaceuticals in plasmaJournal of Chromatography A, 1998
- Use of generic fast gradient liquid chromatography–tandem mass spectroscopy in quantitative bioanalysisJournal of Chromatography B: Biomedical Sciences and Applications, 1998
- The use of turbulent flow chromatography/mass spectrometry for the rapid, direct analysis of a novel pharmaceutical compound in plasmaRapid Communications in Mass Spectrometry, 1997
- The Use of Automated Solid Phase Extraction in the '96 well' Format for High Throughput Bioanalysis using Liquid Chromatography Coupled to Tandem Mass SpectrometryRapid Communications in Mass Spectrometry, 1996
- Introduction to Drug MetabolismPublished by Springer Nature ,1994