Quantitative analysis of neutral glycosphingolipids from human lymphocyte subpopulations

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Abstract
The glycosphingolipids of normal human lymphocytes from individual donors were analyzed by high-pressure liquid chromatography. Purified T- and B-lymphocytes were examined separately. Lactosylceramide was the major neutral glycosphingolipid in human lymphocytes and monohexosylceramide, trihexosylceramide, globoside and paragloboside were all detected in smaller amounts. Analysis of purified B- and T-cell fractions revealed that each of these populations contained a similar qualitative profile for neutral glycosphingolipids, but quantitatively, B cells contained several times more of each glycosphingolipid/cell than did T cells.