Production of lymphokines by circulating human T lymphocytes that express or lack receptors for interleukin 2.

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Abstract
The capacity for circulating human T cells which have or lack receptors for interleukin 2 (IL 2) to produce IL 2, interleukin 3 (IL 3), and interferon-gamma (IFN-gamma) under the stimulus of phytohemagglutinin was studied. By using the monoclonal anti-Tac antibody which reacts against IL 2 receptors on human T cells, concanavalin A-treated T cells were separated into IL 2 receptor-positive (Tac+ T cells) and IL 2 receptor-negative (Tac- T cells) lymphocytes. The results show that Tac+ T cells secreted IL 2 and IFN-gamma but not IL 3. Tac- T cells produced IL 2 and IL 3 but not IFN-gamma. It is concluded that: 1) both T cells lacking and T cells having receptors for IL 2 produce IL 2, but only IL 2 receptor-negative T cells appear to secrete IL 3; and 2) virtually all of the T cells that produce IFN-gamma after PHA stimulation express receptors for IL 2.