Predominance of t cells in the lymphocytic infiltrates of synovial tissues in rheumatoid arthritis

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Abstract
Synovial tissues from 5 patients with rheumatoid arthritis (RA) were examined with immunofluorescence microscopy for the presence of lymphocytes with either bone marrow-derived (B) or thymus-derived (T) surface markers. Five synovial tissues with severe to mild lymphocytic infiltrations by bright field microscopy were examined in parallel with immunofluorescence. B cells were identified with a pepsin-digested fluoresceinated anti-F (ab')2 antiserum and T cells were detected with a specific rabbit anti-T lymphocyte antiserum. By these techniques 75–90% of the lymphocytes in these frozen sections were identified as T cells. Cell suspensions were also prepared by collagenase digestion of two of the five synovial tissues. The lymphocytes in these cell suspensions were predominantly T lymphocytes (78–85%) as shown by their ability to form spontaneous rosettes with sheep erythrocytes (E rosettes).