Codon usage can affect efficiency of translation of genes inEscherichia coli
- 11 September 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (17) , 6663-6671
- https://doi.org/10.1093/nar/12.17.6663
Abstract
By inserting synthetic oligonucleotides into a highly expressed gene in E.coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGC) a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.Keywords
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