The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes
Open Access
- 1 September 1991
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 13 (3) , 171-174
- https://doi.org/10.1111/j.1472-765x.1991.tb00600.x
Abstract
The linear polymerase chain reaction was used to sequence amplified RNA genes from strains of Bacillus, Thermus and Legionella. The technique described is simple and reproducible and it works well with double standard product which has been PEG precipitated directly from PCR reactions.Keywords
This publication has 15 references indexed in Scilit:
- The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regionsPublished by Elsevier ,2003
- Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNAFEMS Microbiology Letters, 1989
- Rapid extraction of bacterial genomic DNA with guanidium thiocyanateLetters in Applied Microbiology, 1989
- Improved double-stranded DNA sequencing using the linear polymerase chain reactionNucleic Acids Research, 1989
- Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.Proceedings of the National Academy of Sciences, 1988
- Polymerase chain reaction reveals cloning artefactsNature, 1988
- [12] Reverse transcriptase sequencing of ribosomal RNA for phylogenetic analysisPublished by Elsevier ,1988
- Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferaseAnalytical Biochemistry, 1986
- Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.Proceedings of the National Academy of Sciences, 1985
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961