Chemical Studies of Partially Hydrolysed Lipopolysaccharides from Four Strains of Campylobacter jejuni and Two Strains of Campylobacter coli

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Abstract
Lipopolysaccharides (LPS) from 4 strains of C. jejuni and 2 strains of C. coli were partially hydrolyzed with 1% acetic acid. Subsequent chloroform extraction led to the formation of a polysaccharide-containing aqueous layer, an interfacial material and a lipid A-containing chloroform layer. The polysaccharides contained the neutral sugars, amino sugars, 2-keto-3-deoxy-octonic acid and part of the P present in the undegraded LPS. The lipid A was made up of glucosamine, P, ester- and amide-linked 3-hydroxytetradecanoic acid, and ester-linked n-tetradecanoic and n-hexadecanoic acid. The interfacial material was made up of lipid A and undegraded LPS. When chromatographed on Bio-Gel P-60, the degraded polysaccharides were eluted as 2 incompletely separated peaks (strains NCTC 11168, NCTC 11351, 11041 and 11101) or as 1 peak (strains NCTC 11392 and E 8035). All peaks appeared close to the total volume of the column. When the different fractions were re-chromatographed on Bio-Gel P-10, the peaks still appeared close to the total volume of the column. Thus, LPS from C. jejuni and C. coli are devoid of long O-antigenic side-chains.

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