Electrophysiological and morphological study of polyneuronal innervation in the cutaneous pectoris muscle of adult frog (Rana esculenta)

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Abstract
Summary The incidence of polyneuronal innervation in the cutaneous pectoris muscle of the adult frog,Rana esculenta, was determined quantitatively using electrophysiological and morphological techniques. The mean percentages of multiple innervated endplates obtained with both techniques from a series of 19 muscles examined at all times of the year were in very good correlation: 30.7% (196/639 endplates) by electrophysiology and 30.5% (478/1569 endplates) by morphology. In nine muscles examined during the period from December to March the mean percentages, 36.8% (110/299) by electrophysiology and 38.6% (281/727) by morphology, were significantly higher than those obtained for 10 muscles investigated during the period from May to November, 25.3% (86/340) and 23.4% (197/842) with both techniques respectively. The higher incidence of collateral sprouted branches detected at polyinnervated endplate sites in muscles of winter frogs might be related to these seasonal variations. Most of the 1688 fibres from 26 muscles examined throughout the year exhibited one centrally located endplate. However, around 11% of them were found to be innervated at two separate endplate sites. Muscle fibres exhibiting this type of innervation were invariably the largest fibres in each muscle tested, having an apparent diameter greater than 48 μm. The distance between the endplates of these fibres represented between 10 and 30% of their total length. No significant seasonal variations were observed in the incidence of these dually innervated fibres. In conclusion, both electrophysiological and morphological results show that the normal incidence of polyneuronal innervation in the cutaneous pectoris muscle of adultRana esculenta is affected by seasonal related factors which influence the nodal sprouting activity. Moreover, they show that a dual pattern of innervation is a common feature in large fibres of this muscle.