Oxidatively modified human low-density lipoprotein stimulates leukocyte adherence to the microvascular endothelium in vivo
- 1 December 1991
- journal article
- Published by Springer Nature in Zeitschrift für Die Gesamte Experimentelle Medizin
- Vol. 191 (1) , 85-90
- https://doi.org/10.1007/bf02576662
Abstract
In vitro studies indicate that oxidatively modified low-density lipoprotein (oxLDL) promotes leukocyte sticking to the vascular endothelium, a constant feature of early atherogenesis. Using intravital fluorescence microscopy in the dorsal skinfold chamber model in hamsters, we investigated whether systemic administration of human LDL, oxidized by Cu2+, elicited leukocyte/endothelium interaction in vivo. While no effect was seen after injection of native LDL, oxLDL administration resulted in an immediate induction of leukocyte rolling along the microvascular endothelium and subsequent firm sticking to the wall of postcapillary venules as well as arterioles. The presented model may provide an alternative experimental approach to long-term feeding studies with atherogenic diets for the in vivo investigation of leukocyte/endothelium interaction in early atherogenesis.Keywords
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