Production and Characterization of Two New Mouse Monoclonal Antibodies Reactive with Denatured Mouse Class II Beta Chains

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Abstract
We report on the production and characterization of two murine peptide specific anti-major histocompatibility complex class II chain specific monoclonal antibodies. Two new mouse monoclonal antibodies reactive with two synthetic peptides corresponding to Abb amino acids (146-157) and Abs amino acids(146-157) were produced. KL295 is the mouse anti-Abb monoclonal antibody, which reacts with denatured β chains of H-2b, H-2d, H-2P, and H-2q, butfail to react with spleen cell lysates from H-2f, H-2j, H-2k and H-2S mice The mouse anti Abs monoclonal antibody, KL304 in contrast reacts with the denatured Class II β chains of H-2f, H-2j, H-2k and H-2S mice, but fails to bind H-2b, H-2d, H-2P or H-2q β chain, suggesting residues 153-155 of this molecule to be critical for the epitope. Both KL295 and KL304 bind B10.WB (H-2j) which suggests a unique epitope in this strain of mice. Neither KL295 or KL304 react with native mouse class II cell surface molecules.