Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase
Open Access
- 20 June 1993
- journal article
- review article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 42 (2) , 251-254
- https://doi.org/10.1002/bit.260420214
Abstract
A subzero temperature operating biosensor was constructed using immobilized quinoprotein glucose dehydrogenase (PQQGDH), glassy carbon electrode, soluble electron mediator (ferrocene monocarboxylic acid), and an organic solvent, ethylene glycol, as an antifreezing reagent. Using this biosensor, glucose concentration can be determined even at −7°C. At this temperature, the response was 20% of that obtained at 20°C. This is the first study describing a subzero temperature operating biosensor. © 1993 John Wiley & Sons, Inc.Keywords
This publication has 11 references indexed in Scilit:
- The structure of bacterial quinoprotein dehydrogenasesInternational Journal of Biochemistry, 1992
- Preliminary studies on quinoprotein glucose dehydrogenase under extreme conditions of temperature and pressureBiochimie, 1991
- Microbial BOD Sensor Utilizing Thermophilic BacteriaAnalytical Letters, 1989
- Temperature profiles of biogas plants operating under hilly conditionsBiological Wastes, 1989
- Psychrophilic methane fermentation of excess sludge by enrichment cultureJournal of Fermentation Technology, 1988
- [47] Enzyme studies at subzero temperatures using immobilized substratesPublished by Elsevier ,1987
- Isolation and temperature characterization of psychrotrophic strains of Thiobacillus ferrooxidans from the environment of a uranium mine.The Journal of General and Applied Microbiology, 1986
- Supercooled water as medium for enzyme reactions at subzero temperaturesBiochimica et Biophysica Acta (BBA) - Enzymology, 1978
- Glucose Dehydrogenase of Bacterium anitratum: an Enzyme with a Novel Prosthetic GroupJournal of Biological Chemistry, 1964