Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system
Open Access
- 1 October 1992
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (20) , 6685-6687
- https://doi.org/10.1128/jb.174.20.6685-6687.1992
Abstract
D55Q-T83A and D55Q-G94S, two pseudorevertants of the D55Q mutant OmpR, an Escherichia coli transcriptional activator, were isolated previously by R. Brissette, K. Tsung, and M. Inouye (J. Bacteriol. 173:3749-3755, 1991). These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment. These proteins were transcriptionally active, even after acid treatment, whereas the wild-type OmpR was completely inactive after the same treatment. Phosphorylation of acid-treated wild-type OmpR with an EnvZ11 membrane fraction and ATP restored transcriptional activity, whereas the activities of the mutant OmpR proteins did not change after phosphorylation.Keywords
This publication has 24 references indexed in Scilit:
- Intermolecular complementation between two defective mutant signal-transducing receptors of Escherichia coli.Proceedings of the National Academy of Sciences, 1991
- Substitutions at a single amino acid residue in the nitrogen‐regulated activator protein NTRC differentially influence its activity in response to phosphorylationMolecular Microbiology, 1991
- Signal transduction and osmoregulation in Escherichia coli. A novel type of mutation in the phosphorylation domain of the activator protein, OmpR, results in a defect in its phosphorylation-dependent DNA bindingJournal of Biological Chemistry, 1991
- Phosphorylation of a bacterial activator protein, OmpR, by a protein kinase, EnvZ, stimulates the transcription of the ompF and ompC genes in Escherichia coliFEBS Letters, 1990
- Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.Proceedings of the National Academy of Sciences, 1990
- Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor.Genes & Development, 1989
- Phosphorylation of OmpR by the osmosensor EnvZ modulates expression of the ompF and ompC genes in Escherichia coli.Proceedings of the National Academy of Sciences, 1989
- A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.Genes & Development, 1989
- Environmentally Regulated Gene Expression for Membrane Proteins in Escherichia coliAnnual Review of Cell Biology, 1988
- Mechanism for the autogenous control of the crp operon: transcriptional inhibition by a divergent RNA transcript.Proceedings of the National Academy of Sciences, 1986