Organisation of the genes encoding glycerol dehydratase of Lactobacillus collinoides, Lactobacillus hilgardii and Lactobacillus diolivorans

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Abstract
Lactobacillus collinoides IŒB 9527, Lactobacillus hilgardii IŒB 0003 and strain IŒB 0004 belonging to the recently described Lactobacillus diolivorans species which could degrade glycerol in anaerobic conditions were studied. Two degenerated primers, deduced from Citrobacter freundii, Klebsiella pneumoniae, Klebsiella oxytoca, Salmonella typhimurium and Clostridium pasteurianum glycerol dehydratase sequence alignments, were used for polymerase chain reaction amplification. The three 279 base pair amplicons from L. collinoides, L.hilgardii and L. diolivorans showed high homologies with part of the glycerol dehydratase genes already sequenced. The three amplicon DNA nucleotide sequences were used to design specific primers for inverse-PCR in order to study the entire glycerol dehydratase genes of the three species. Amplified fragments were cloned and then sequenced, several open reading frames were identified. For the three species, the genetic organization of the glycerol degradation genes suggested operons. Some deduced proteins were very similar to the carbon dioxide concentrating protein family. The others are three subunits similar to adenosylcobalamin dependent glycerol or propanediol dehydratases respectively from C. freundii, K. pneumoniae and K. oxytoca and S. typhimurium.

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